Research Department:
Dr. A. Jesu Arockia Raj, Professor of Life Sciences division at SRM Research Institute and associated with the Department of Biotechnology of SRM Institute of Science and Technology, since June 2012. He received his doctoral degree from Manonmaniam Sundaranar University, Tirunelveli, India, where he established himself as a fisheries biotechnologist. Then, he moved to different Universities between 2003 and 2012 for his postdoctoral research. He developed his experience on fish biochemistry especially on fatty acid metabolism and regulation in marine fishes at Shantou University, China. At Ben-Gurion University of the Negev, Israel he established such a system of desert aquaculture engineering to culture marine fishes in deserts. Further, at Jeju National University, South Korea followed by University of Malaya, Malaysia he concentrated on construction of cDNA libraries of varies commercially important aquatic organisms including fish, shellfish and aquatic algae. Presently, at SRMIST he focused on development of transcriptome database for aquatic organisms to address the immunological parameters including cloning, sequencing, molecular characterization, gene expression, recombinant protein expression and purification.
Academic Profile
- Ph. D (2002) Manonmaniam Sundaranar University, Tirunelveli, India
- M.Phil (1998) Manonmaniam Sundaranar University, Tirunelveli, India
- M. Sc (1997) Manonmaniam Sundaranar University, Tirunelveli, India
- B.Sc (1995) Manonmaniam Sundaranar University, Tirunelveli, India
Employment details
- Professor (June 2018 - till date), SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, India
- DBT- Ramalingaswami Fellow and Assoc. Professor (2012-2018) Dept. of Biotechnology, Faculty of Science and Humanities, SRMIST, Kattankulathur
- Postdoctoral Fellow (2010-2012) Institute of Biological Sciences, Faculty of Science, University of Malaya, Malaysia
- Postdoctoral Research Associate (2009-2010) Dept. of Biotechnology, School of Bio-Medical Sciences, Jeju National University, South Korea
- Postdoctoral Fellow (2007-2009) The Jacob Blaustein Institute for Scientific Cooperation, Ben-Gurion University of the Negev, Israel
- Research Associate (2005-2007) Jovika Media Pvt. Ltd, Chennai, India
- Postdoctoral Fellow (2003-2005) Department of Biology, Shantou University, China
Awards & Recognitions
- Tamil Nadu Young Scientist Award 2014 in Biological Sciences, Academy of Science, Chennai
- Young Scientist, Science and Engineering Research Board, Department of Science & Technology, Government of India, New Delhi.
- DBT Prestigious Ramalingaswami Re-entry Fellowship, Department of Biotechnology, Government of India, New Delhi
- Distinguished Bright Spark Fellowship, University of Malaya, Malaysia
- BK 21 Postdoctoral Research Fellowship, Jeju National University, South Korea
- Jacob Blaustein Postdoctoral Fellowship, Ben-Gurion University of the Negev, Israel
- Budding Scientist, All India Radio, Tirunelveli
- Indian Council of Agricultural Research (ICAR) Research Fellowship, Ministry of Agriculture, New Delhi
- Tamil Nadu State Council of Science and Technology Project Fellowship, Directorate of Collegiate Education, Chennai
- Indian Science Congress Association, Kolkata, India
Membership
- Society of Fisheries Technologists (SOFT), Cochin, India
- Society of Aquaculture Professionals (SAP), Chennai, India
- Indian Nano-Biologists Association (INBA), Chennai, India
- Construction of transcriptome dataset for freshwater prawn Machrobrachium rosenbergii, freshwater fish striped murrel Channa striatus, marine fish rock bream Oplegnathus fasciatus, marine flatfish olive flounder Paralichthys olivaceus, marine shellfish Manila clam Venerupis philippinarum, Spirulina Spirulina platensis and Fungus Aphanomyces invadans.
- Identification, isolation, sequencing and bio-informatics characterization of the immune related genes from the transcriptome.
- Studies on the relative gene expression in various tissues of the above said experimental animals using quantitative real time PCR. Studies on the mRNA expression and transcriptional analysis upon microbial infection.
- Cloning the coding sequence of the immune genes and purify the recombinant protein to study the gene specific protein activity analysis.
- Identification of short peptides (antimicrobial and antioxidant) using bioinformatics tools from trancriptome database and synthesis the peptides to study its various activities.
- So far, 107 defense genes have been identified and sequenced from different commercially important finfish and shellfish for molecular characterization studies; and the sequences were submitted to NCBI and EMBL nucleotides databases under various GenBank Accession IDs.
Research Area
- Bioinformatics
- Molecular Biology
- Immunology
- Antimicrobial Peptides
Major Research Facilities Available
- Cell culture unit
- Gene expression studies (qRT-PCR)
- 2D gel Electrophoresis
Research projects
S.No | Title of the project | Funding Agency & Reference No. | Role in the Project | Status | Amount (Rs. in lakh) |
1. | cDNA profiling in targeting novel immune related genes in epizootic ulcerative syndrome (EUS) infected striped murrel Channa striatus | Department of Biotechnology, Government of India, New Delhi
|
PI | Completed (June 2012-May 2018) |
101.9 |
2. | Molecular and proteomic identification of immune related genes in freshwater prawn Macrobrachium rosenbergii | Science & Engineering Research Board (SERB), Department of Science & Technology, Government of India, New Delhi
|
PI | Completed (August 2012- July 2015) |
18.1 |
3. | Virulence factors identification in fish disease [epizootic ulcerative syndrome (EUS)] causing fungus Aphanomyces invadans by transcriptome approach | Department of Biotechnology, Government of India, New Delhi
| PI | On-going (October 2016- September 2019)
| 36.952 |
4. | Characterization of cellular and molecular mechanisms involved in fish lectins and its potential role in innate immune defense and therapeutics | Science & Engineering Research Board (SERB), Department of Science & Technology, Government of India, New Delhi
| Co-PI | On-going (November 2016- October 2019)
| 39.8552 |
5. | 102nd EC meeting on Science and Technology for Women
| Science for Equity Empowerment and Development Division, Department of Science & Technology, Government of India | Coordinator | Completed (April 2016- March 2017) | 10.29 |
Patents Filed
- Jesu Arockiaraj (2014) Delivering live spirulina jelly as fish feed supplement: An eco-friendly integrated aquaculture approach. Appl. No. 2362/CHE/2014 dt. 12/05/2014
Group Leader
Dr. A. Jesu Arockia Raj
Ph.D students - 5
Gayathri R. Chandran - Isolation, identification and molecular characterization antimicrobial peptides from murrel skin mucus
Faizal, N - Isolation, identification and molecular characterization of proteins from blue green algae spirulina, Arthrospira platensis
Anbazahan, S - Molecular characterization of selected antioxidant genes from sprirulina and determination of its biological activities
Prabha N - Exploration of antimicrobial peptides (AMPs) from innate immune molecules of snakehead fish
Kasi Viswananthan, M - Development of Feed Additives and their Performances for Sustainable Aquaculture Practice
Postdoctoral fellow - 1
Venkatesh, K - Identification and bioinformtics characterization of virulent factors from fish disease causing fungus Apahnomyces invadans
Ph. D completed - 6
Prashanth Bhatt, N - Gene profiling and characterization of chemokines from snakehead murrel Channa striatus
Rajesh, P - Molecular functions of selected disease related genes from Channa striatus
Venkatesh, K - Molecular cloning, characterization and gene expression of defense genes from striped murrel Channa striatus challenged with various immune stimulants
Abirami, K - Fish antimicrobial peptides: Bioinformatic characterization, gene expression and recombinant protein activities
Akila, S - Gene expression and biological activities of immune genes from Channa striatus infected with fungus (Aphanomyces invadans) and bacteria (Aeromonas hydrophilla)
Mukesh Kumar Chaurasia - Gene expression and characterization of a few immune genes from fresh water prawn (Marcobrachium rosenbengii)
Total No. of Publications : 73
- Total Impact Factor :229.774 (as on 8 June 2018)
- Citation Number :1379 (as on 8 June 2018)
- H-Index : 20 (as on 8 June 2018)
Chapters in Books : 4
Other General & Semi-Scientific Articles in Popular Journal/Magazines : 58
Papers Presented in International Conferences : 46
Papers Presented in National Conferences : 28
Attended Workshops/Training Programs : 19
Invited Talks : 11
Programs Conducted : 5
* in the publications denotes the designation of Corresponding Author
Selected Publications
- Tripathi VC, Sabbu S, Horam S, Raj S, Lal A, Arockiaraj J, Pasupuleti M, Dikshit DK (2018) Natural products from polar organisms: structural diversity, bioactivities and potential pharmaceutical applications. Polar Science DOI: 10.1016/j.polar.2018.04.006 (in press) (IF = 1.118)
- Rajesh P, Prasanth B, Venkatesh K, Mukesh P, Arockiaraj J* (2018) Innate and adaptive immune molecules of striped murrel Channa striatus. Reviews in Aquaculture DOI: 0.1111/raq.12161 (in press) (Impact factor = 4.618)
- Gayathri R, Venkatesh K, Mahesh A, Dhayalan A, Arshad A, Arasu MV, Al-Dhabi NA, Pasupuleti M, Arockiaraj J* (2018) Bactericidal and fungistatic activity of peptide derived from GH18 domain of prawn chitinase 3 and its immunological functions during biological stress. International Journal of Biological Macromolecules 106: 1014-1022 (impact Factor = 3.671)
- Venkatesh K, Rajesh P, Mukesh P, Arockiaraj J*(2017) Impacts of environmental and biological stressors on immune system of Macrobrachium rosenbergii. Reviews in Aquaculture 9: 283-307 (Impact factor = 4.618)
- Venkatesh K, Faizal N, Gayathri R, Kasi V, Rajesh P, Prasanth B, Arasu MV, Al-Dhabi NA, Kanchana M, Arockiaraj J* (2017) Transcriptome changes of Blue-Green algae, Arthrospira sp in response to sulfate stress. Algal Research 23: 96-103 (Impact factor = 3.994)
- Soniya C, Vijay R, Arokiaraj J, Kanchana M (2017) Caveolin1/Protein Arginine Methyltransferase1/Sirtuin1 Axis as a Potential Target Against Endothelial Dysfunction. Pharmacological Research 119: 1-7 (Impact Factor = 4.480)
- Abirami A, Venkatesh K, Ganesh MR, Mukesh P, Arasu MV, Al-Dhabi NA, Arockiaraj J* (2017) Bactericidal activity of fish galectin 4 derived membrane-binding peptide tagged with oligotryptophan. Developmental and Comparative Immunology 71: 37-48 (Impact Factor = 3.218)
- Akila S, Chaurasia MK, Arasu MV, Al-Dhabi NA, Harikrishnan R, Arockiaraj J* (2017) Differences in structure and changes in gene regulation of murrel molecular chaperone HSP family during epizootic ulcerative syndrome (EUS) infection. Fish and Shellfish Immunology 60: 129-140 (Impact Factor = 3.148)
- Anju T, Preetha R, Shunmugam R, Mane SR, Arockiaraj J, Venkatesh K(2016) Norbornene derived nanocarrier reduces isoniazid mediated liver toxicity: assessment in HepG2 cell line and zebrafish model. RSC Advances 6: 114927-114936 (Impact Factor = 3.108)
- Abirami A, Venkatesh K, Rajesh P, Arasu MV, Al-Dhabi NA, Ganesh MR, Arockiaraj J* (2016) Bacterial membrane binding and pore formation abilities of carbohydrate recognition domain of fish lectin. Developmental and Comparative Immunology 67: 202-212 (Impact Factor = 3.218)
- Gayathri R, Venkatesh K, Arasu MV, Al-Dhabi NA, Ganesh MR, Arun M, Dhayalan A, Mukesh P, Arockiaraj J* (2016) Pellino-1 derived cationic antimicrobial prawn peptide: Bactericidal activity, toxicity and mode of action. Molecular Immunology 78: 171-182 (Impact Factor = 3.236)
- Mukesh KC, Faizal N, Gayathri R, Arasu MV, Al-Dhabi NA, Aziz A, Elumalai P, Jesu Arockiaraj* (2015) Molecular importance of prawn large heat shock proteins 60, 70 and 90. Fish and Shellfish Immunology 48: 228-238 (Impact Factor = 3.148)
- Venkatesh K, Prasanth B, Ganesh MR, Harikrishnan R, Arasu MV, Al-Dhabi NA, Mukesh P, Marimuthu K, Jesu Arockiaraj* (2015) A novel antimicrobial peptide derived from fish goose type lysozyme disrupts the membrane of Salmonella enterica. Molecular Immunology 68: 421-433 (Impact Factor = 3.236)
- Jesu Arockiaraj*, Prasanth B, Harikrishnan R, Arasu MV, Al-Dhabi NA (2015) Molecular and functional roles of 6C CC chemokine 19 in defense system of striped murrel Channa striatus. Fish and Shellfish Immunology 45: 817-827 (Impact Factor = 3.148)
- Venkatesh K, Annie JG, Mukesh P, Mariadhas VA, Al-Dhabid NA, Harikrishnan R, Arockiaraj J* (2015) Comparative analysis of CsCu/ZnSOD defense role by molecular characterization: Gene expression-Enzyme activity-Protein level. Gene 564: 53-62 (Impact Factor = 2.415)
- Rajesh P, Venkatesh K, Harikrishnan R, Arasu MV, Al-Dhabi NA, Arockiaraj J* (2015) Functional roles and gene regulation of tumor necrosis factor receptor 1 in freshwater striped murrel. Molecular Immunology 66: 240-252 (Impact Factor = 3.003)
- Arockiaraj J*, Palanisamy R, Arasu A, Sathyamoorthi A, Kumaresan V, Bhatt P, Chaurasia MK, Pasupuleti M, Annie JG (2015) An anti-apoptotic B-cell lymphoma-2 (BCL-2) from Channa striatus: Sequence analysis and delayed and advanced gene expression in response to fungal, bacterial and poly I:C induction. Molecular Immunology 63: 586-594 (Impact Factor = 3.236)
- Arockiaraj J*, Palanisamy R, Bhatt P, Kumaresan V, Annie JG, Pasupuleti M, Marimuthu K (2014) A novel murrel Channa striatus mitochondrial manganese superoxide dismutase: Gene silencing, SOD activity, superoxide anion production and expression. Fish Physiology and Biochemistry 40: 1937-1955 (Impact Factor = 1.647)
- Arasu A, Kumaresan V, Sathyamoorthi A, Chaurasia MK, Bhatt P, Annie JG, Palanisamy R, Marimuthu K, Pasupuleti M, Arockiaraj J* (2014). Molecular characterization of a novel proto-type antimicrobial protein galectin-1 from striped murrel. Microbiological Research 169: 824-834 (Impact Factor = 3.037)
- Arockiaraj J*, Sathyamoorthi A, Kumaresan V, Palanisamy R, Chaurasia MK, Bhatt P, Annie JG, Pasupuleti M, Arasu A (2014) A murrel interferon regulatory factor-1: Molecular characterization, gene expression and cell protection activity. Molecular Biology Reports 41: 5299-5309 (Impact Factor = 1.958)
- Arockiaraj J*, Annie JG, Palanisamy R, Bhatt P, Kumaresan V, Chaurasia MK, Pasupuleti M, Harikrishnan R, Arasu A, Sathyamoorthi A (2014) A cytosolic glutathione s-transferase, GST-theta from freshwater prawn Macrobrachium rosenbergii: Molecular and biochemical properties. Gene 546: 437-442 (Impact Factor = 2.415)
- Thirumalai MK, Roy A, Sanikommu S, Arockiaraj J, Pasupuleti M (2014) A simple, robust enzymatic-based high-throughput screening method for antimicrobial peptides discovery against Escherichia coli. Journal of Peptide Science 20: 341-348 (Impact Factor = 1.972)
- Arockiaraj J*, Kumaresan V, Bhatt P, Palanisamy R, Annie JG, Pasupuleti M, Marimuthu K, Chaurasia MK (2014) A novel single-domain peptide, anti-LPS factor from prawn: Synthesis of peptide, antimicrobial properties and complete molecular characterization. Peptides 53: 79-88 (Impact Factor = 2.778)
- Bhatt P, Kumaresan V, Palanisamy R, Mukesh KC, Annie JG, Pasupuleti M, Arockiaraj J* (2013) Immunological role of C4 CC chemokine-1 from snakehead murrel Channa striatus. Molecular Immunology 57: 292-301 (Impact Factor = 3.236)
- Arockiaraj J*, Annie JG, Palanisamy R, Kumaresan V, Bhatt P, Thirumalai MK, Roy A, Pasupuleti M, Marimuthu K, Sathyamoorthi A, Arasu A (2013) A prawn transglutaminase: Molecular characterization and biochemical properties. Biochimie 95: 2354-2364 (Impact Factor = 3.112)
- Marimuthu K, Narmataa M, Rathinam X, Arockiaraj J, Rahman MA, Sreeramanan S (2013) Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch) PLoS ONE 8: e75545 (Impact Factor = 2.806)
- Arockiaraj J*, Annie JG, Muthukrishnan D, Thirumalai MK, Pasupuleti M, Milton J, Marimuthu K (2013) Macrobrachium rosenbergii Cathepsin L: Molecular characterization and gene expression in response to viral and bacterial infections. Microbiological Research 168: 569-579 (Impact Factor = 3.037)
- Arasu A, Kumaresan V, Sathyamoorthi A, Palanisamy R, Prabha N, Bhatt P, Roy A, Thirumalai MK, Annie JG, Pasupuleti M, Marimuthu K, Arockiaraj J* (2013) Fish lily type lectin-1 contains β-prism 2 architecture: Immunological characterization. Molecular Immunology 56 (4): 497-506. (Impact Factor = 3.236)
- Arockiaraj J*, Annie JG, Dhanaraj M, Pasupuleti M, Milton J, Singh A (2013) An upstream initiator caspase 10 of snakehead murrel Channa striatus, containing DED, p20 and p10 subunits: Molecular cloning, gene expression and proteolytic activity. Fish and Shellfish Immunology 34: 505-513 (Impact Factor = 3.236)
- Arockiaraj J, Vanaraja P, Easwvaran S, Singh A, Othman RY, Bhassu S (2012) Molecular functions of chaperonin gene, containing tailless complex polypeptide 1 from Macrobrachium rosenbergii. Gene 508: 241-249 (Impact Factor = 2.415)
- Animal Physiology
- Environmental Biotechnology
- Cancer Biology
- Industrial Biotechnology